Blood And Tissues

TITLE: BLOOD & TISSUES- Staining Of Blood Films & Identification Of Leukocytes. OBJECTIVES: i.To assure the preparation of beginning films from human blood sample. ii.To learn the techniques of dishonor the blood films with Giemsa filthiness and Wright territory. iii.To identify and make drawing of leukocytes that bribe in human blood sample under microscope. interpellation: Leukocytes be divided into granulocytes and agranulocytes. Granulocytes argon also known as polymorphonuclear leukocytes, or polys for short. Three types of granulocyte are neutrophils, bromeosinophils, basophils. Meanwhile, there are ii types of agranulocyctes which are lymphocytes and monocytes. If we observe the smear as it is after fixing, we pass on gibe really little because all cells are very transparent. The erythrocytes are slightly visible, but the leukocytes are in any case pale, just about invisible and we will not see anything inside them. To be able to observe and rec ognize the antithetical kinds of leukocyte, we must dirt them. For this purpose, normally Giemsa speckle and Wright stain are used. Giemsa stain is a mixture of dyes, based on methylene group blue (basic dye) and eosin ( virulent dye). Giemsa stain is a classical blood film stain for peripheral blood smears and bone bone marrow specimens.

The principle of Giemsa stain is the basic dye stain the acidic sectionalisation of the burden of the cell and the acidic dye stained the basic part of the cytoplasm of the cell. Therefore, the Giemsa stain allows clear observations to assure types of leukocytes. By patch of the cells we can observe the different t! ypes of unobjectionable blood cells (leukocyctes) which are, neutrophils, eosinophils, basophils, lymphocytes and monocytes under the light microscope. MATERIALS: 1)Alcohol swabs 2)Blood Lancets 3)Deionized water 4)Distilled water 5)Giemsa stain 6)Glass Slides 7)Jar of disinfection for disposal of slides 8)Methanol 9)Microscopes 10)Oil Immersion 11)Pasteur pipettes 12)Sorensens...If you want to get a broad(a) essay, fix it on our website: OrderCustomPaper.com

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